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FAM-FLICA® Caspase-1 (YVAD) 检测试剂盒是ImmunoChemistry公司开发的一款检测试剂盒，该产品采用荧光抑制剂探针FAM-YVAD-FMK标记活细胞中的活性caspase-1酶。
                           
 

研究认为，Caspase在细胞凋亡和炎症中通常起重要作用。而ICT开发的这款检测试剂盒就可以用来检测caspase活性来定量细胞凋亡。FAM FLICA Caspase-1探针能够检测Caspase-1是否处于活化状态。FLICA试剂FAM-YVAD-FMK能够进入每个细胞并且会不可逆地与活化的caspase-1结合。FAM-YVAD-FMK FLICA试剂会共价偶联活性酶，使得它保留在细胞内，而没有结合的FAM-YVAD-FMK FLICA试剂则会被洗脱。通过绿色荧光信号可以对细胞中存在的活性caspase-1酶活性进行直接测量。含有结合FLICA的细胞可以通过酶标仪、荧光显微镜或流式细胞术进行分析。

• FAM-FLICA的最佳激发波长为488-492nm，峰值发射波长为515-535nm。

• 在长通滤波器下观察碘化丙啶(PI)，激发波长为488-492nm，发射波长为610nm；核结合的PI的最大发射波长为617nm。

• 使用紫外滤光片来观测Hoechst 33342，激发波长为365nm，发射波长为480nm。
                     
             
              
   

  参考文献

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  [4].Cui, Y;Yang, Y;Tao, W;Peng, W;Luo, D;Zhao, N;Li, S;Qian, K;Liu, F. Neutrophil Extracellular Traps Induce Alveolar Macrophage Pyroptosis by Regulating NLRP3 Deubiquitination, Aggravating the Development of Septic Lung Injury. Journal of Inflammation Research. 2023 February

  [5].Ramadan, A;Cao, Z;Gadjeva, M;Zaidi, TS;Rathinam, VA;Panjwani, N. The NLRP3 Inflammasome Is Required for Protection Against Pseudomonas Keratitis. Investigative ophthalmology & visual science. 2023 February

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